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In this chapter, we describe protocols for the isolation of adecs from fresh and nuclei from frozen samples For more information, see the developer’s privacy policy. Here, we delineate a protocol for the acquisition of microvascular endothelial cells from human fat.
Here, we present a protocol for the isolation of endothelial cells (ecs) from tissues. The company owes its success to experimentation and innovation and invites. Compared with previously published protocols, we illustrate its applicability across species and different types of adipose tissue (visceral and subcutaneous) and highlight critical points such as the importance of measuring collagenase enzymatic activity when adding to the adipose tissue digestion suspension.
This protocol details a method for the dissection of mouse adipose depots and the isolation and digestion of respective arteries to liberate and then identify the endothelial cell population.
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